Table of Content

15 February 2017, Volume 44 Issue 1
Previous Issue    Next Issue

---

For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

Select

Research of DNA methylation of microRNA in ovarian cancer Lei-Lei NIU 2017, 44 (1):  9-12.  Abstract ( 1368 )   PDF (683KB) ( 7008 )   DNA methylation is one of the most widespread research in epigenetic about tumor. Recently, it has become clear that miRNA play an important role in ovarian cancer pathogenesis, metastasis, drug resistance, and prognosis. The DNA methylation of miRNA in ovarian cancer may be used as a noninvasive diagnostic and prognostic biomarker in ovarian cancer, and provide a new clinical thought for the analysis of drug resistance and individualized therapy. Related Articles | Metrics

Select

Therapy for Elderly Patients With Ovarian Cancer. 2017, 44 (1):  13-17.  Abstract ( 2156 )   PDF (737KB) ( 9748 )   Epithelial ovarian cancer is a cancer of older women. Close to half (45%) of women diagnosed with ovarian cancer will be older than age 64. This ratio is expected to increase in the coming decades as our population ages and life-expectancy improves, while Older women with ovarian cancer receive less surgery and chemotherapy, suffer worse toxicity from surgery and chemotherapy，and have worse survival. The majority of elderly patients are able to tolerate the standard of care for ovarian cancer including initial surgical cytoreduction followed by platinum and taxane chemotherapy. To offer optimal radical management，Pretreatment assessment is needed. Large prospective clinical trials involving elderly ovarian cancer patients are warranted to offer justified evidenced based optimal treatment for those who will be benefited from the treatment. Related Articles | Metrics

Select

Current status of diagnosis and treatment of borderline ovarian tumors 2017, 44 (1):  18-22.  Abstract ( 2084 )   PDF (719KB) ( 9885 )   Abstract：Borderline ovarian tumors (BOT) is a low malignant potential ovarian tumors，it has the characteristics of early onset, early detection and good prgnosis, but the preoperative diagnosis is difficult. Although the serum tumor markers, pelvic ultrasound and intraoperative frozen biopsy could assist the diagnosis, but the accuracy is low. In recent years, the surgical treatment of BOT gradually changed from radical operation to fertility preserving surgery. However, there has been widespread controversy about its early diagnosis, therapy and prognosis, this article reviews the research status of BOT diagnosis and treatment, in order to provide references for the clinical diagnosis and treatment of BOT and new treatment technology research. Related Articles | Metrics

Select

Progress of Exosome in the Pathogenesis, Diagnosis and Treatment of Ovarian Cancer Zhang Tao 2017, 44 (1):  23-26.  Abstract ( 1341 )   PDF (725KB) ( 7209 )   【Abstract 】Ovarian cancer is the highest mortality rate of malignant tumor in gynecologic cancer. Due to the lack of specific markers, the early diagnosis of ovarian cancer is very difficult. In recent years, The exosome has become a hot research topic in many fields，as for it’s widely distributed，rich content and functional diversity，is more and more into the field of cancer research. Exosomes can be derived from many kinds of cells and body fluid. They can mediate cell - cell information exchange, promote the occurrence and development of tumors through the delivery of a variety of proteins, lipids, mRNA and miRNA fragments and other biological functions. In addition, due to the specific antigen of tumor related, exosomes provide a new idea for the diagnosis and treatment of tumors. This article reviews research on the diagnosis and treatment of exosomes in the development and occurrence of ovarian cancer, the mechanism is more precise and more comprehensive in order to reveal the exosomes in ovarian cancer, and provide a theoretical basis for the new treatment methods. Related Articles | Metrics

Select

Research advances in ovarian teratoma associated with anti-N-methyl-D-aspartate receptor encephalitis Jing ZENG 2017, 44 (1):  27-30.  Abstract ( 1143 )   PDF (696KB) ( 6903 )   Abstract: Anti-N-methyl-D-aspartate receptor encephalitis, which is always associated with germ cell tumors such as teratoma, is an autoimmune encephalitis discovered recently. The pathogenesis is high likely related to anti-N-methyl-D-aspartate antibody, which have obstruct the transmission of neural signals. Its clinical manifestation is diverse and the treatment combines immunotherapy with tumor resection. The prognosis is also good. This review mainly introduce the researches on anti-N-methyl-D-aspartate receptor encephalitis associated with ovarian teratoma to improve the awareness of clinician on this recent discovered disease. Related Articles | Metrics

Select

Role of hysteroscopy and laparoscopy in diagnosis and management of gestational trophoblastic neoplasia 2017, 44 (1):  31-34.  Abstract ( 1062 )   PDF (751KB) ( 7256 )   Gestational trophoblastic neoplasia(GTN) usually occurs in women of reproductive age. It is very sensitive to chemotherapy with a high cure rate of 90%-100%.However,in clinical practice,it is very easy to confuse GTN with many other pregnancy-associated diseases,such as ectopic pregnancy. Hysteroscopy and laparoscopy have an important role in the differential diagnosis of GTN，especially for those uterine cavity lesion with normal HCG.They also can effectively salvage some patients with isolated foci of chemoresistant disease, including resecting the GTN lesion and reducing the total courses of chemotherapy. Related Articles | Metrics

Select

The progress of the relationship between cancer stem cells and Endometrial cancer. Ya-Ting zou 2017, 44 (1):  35-39.  Abstract ( 1170 )   PDF (748KB) ( 7003 )   Endometrial carcinoma, which is one of the most common gynecologic malignant tumors, severely threatens women’s health, and its incidence is showing an increasing trend in recent years. Cancer stem cells are defined as a subset of cancer cells with abilities of self-renewal, unlimited proliferation, multipotential differentiation and high tumorigenicity. Current emerging evidences indicate that the cancer stem cells are likely responsible for the development, progression, metastasis and recurrence of endometrial carcinoma. These may be the main reasons for the poor prognosis. This review is a conclusion of the sources and characters of cancer stem cells, and their special surface markers in endometrial carcinoma. Related Articles | Metrics

Select

Research progress of large cell neuroendocrine carcinoma of the uterine cervix Jun-Min LI Jing-Chun Gao 2017, 44 (1):  40-43.  Abstract ( 2437 )   PDF (707KB) ( 7394 )   Large cell neuroendocrine carcinoma ( LCNEC ) of the uterine cervix is a rare but highly aggressive malignancy that tends to spread and recur early even in its early stage. The prognosis is very poor despite multimodal treatments. Neuroendocrine carcinomas sometimes are infected with high-risk human papillomavirus, but they are the subtype of cervical cancer that does not appear to be associated with human papillomavirus ( HPV ) exposure. The overwhelming majority of available data related to its treatment derive from retrospective analyses or small case series. Large-cell neuroendocrine carcinoma seem to behave similar to their more common counterpart, small-cell carcinoma, while large-cell carcinomas are extremely more rare and have a exceedingly different histologic appearance. Because of their peculiar clinical and biological features and the lack of literature data, there is an urgent need for a consensus on the best treatment approach for LCNEC and for the identification of new therapeutic options. The objective of this paper is to review the limited information available about the LCNEC and the diagnosis and treatmen. Related Articles | Metrics

Select

The mechanism of proteasome inhibitor MG132 induces ovarian carcinoma cells autophagic and apoptotic 2017, 44 (1):  44-47.  Abstract ( 1433 )   PDF (1014KB) ( 7254 )   Aim: To observe the growth of ovarian cancer SKOV3 cells after treated with proteasome inhibitor MG132, and the of autophagic and apoptotic factors, to investigate the inhibited mechanism of MG132?affected on ovarian?cancer cells. Method:Observation of SKOV3 cells after treated with MG132 at the concentrations of 0.5μg/ml, 1.5μg/ml, 2.5μg/ml, 3.5μg/ml. The growth of?cells were detected by MTT assay after treated with MG132 ; Apoptotic rates of cells were detected by flow cytometry(FCM) after treated with MG132; The of autophagic and apoptotic factors in cells were detected by IHC, western blot and RT-PCR. Results: MTT assay demonstrated the growth of SKOV3 cells were inhibited after treated with MG132 with concentration and time dependent.;FCM demonstrated the apoptotic rates?were gradually increased with the??increased MG132 concentrations and time.IHC?detected Beclin1 and Caspase3 were positive; Western blot detected Caspase3, Bim, Bax, Beclin1 were high expressed and Bcl-2 was low expressed in SKOV3 cells after treated with MG132, the protein of Beclin1, Caspase3, Bim, Bax were increased with the?increased MG132 concentrations and positive correlation, the protein of Bcl-2 was reduced and negative correlation with concentrations dependent; RT-PCR detected the mRNA relative quantity of Beclin1 and Caspase3 in MG132 groups were all higher than the control group ( P＜0.05 ) .Conclusion: The growth of ovarian carcinoma SKOV3 cells can be inhibited by proteasome inhibitor MG132 with concentration and time dependent. The inhibition effect is not only related with apoptosis, but also with autophagy. Related Articles | Metrics

Select

Anti-tumor effect of Taxotere on nude mice xenografts of human ovarian cancer SKOV3 cells and its synergistic effect of action with Cisplatin，Herceptin 2017, 44 (1):  48-51.  Abstract ( 1490 )   PDF (842KB) ( 7076 )   Objective：1.To study the anti-tumor effect of taxol drugs Taxotere on nude mice xenografts of human ovarian cancer SKOV3 cells and explore its possible mechanism; 2.To compare the anti-tumor effect of Taxotere with the combination of DDP,Herceptin on nude mice xenografts of human ovarian cancer SKOV3 cells.Methods：An animal model with human ovarian cancer SKOV3 cells involved in nude mice was established and the mice were randomized into 8 groups: ①DDP group,②Herceptin group,③Taxotere group,④DDP plus Herceptin group,⑤DDP plus Taxotere group,⑥Herceptin plus Taxotere group,⑦DDP plus Herceptin plus Taxotere group,⑧NS group，There are 4 mice in every group. The mice were administrated respectively with Cisplatin(3mg/kg)、Herceptin (20mg/kg)、Taxotere（5mg/kg） via caudal vein injection once a week for consecutive six weeks. The size of the tumor and mice weight were measured weekly, then the mice were killed a week after last treatment. The inhibition ratio of tumor growth was calculated and tumor tissues were observed by using HE staining. The apoptotic index was analyzed by using Tunel technique. The Ki-67 in xenograft tumors were analyzed by using immunohistochemical staining.Results：Taxotere can significantly inhibit the growth of xenografts of human ovarian cancer SKOV3 cells in nude mice, and the inhibition effect is more prominent when it was joint with Cisplatin or Herceptin respectively, while the inhibition effect is the most obvious when the three drugs above is joint together; 2. The tumor cell apoptotic index of Taxotere group was significantly increased than the control group, and the apoptotic index is higher when Taxotere was joint with Cisplatin or Herceptin respectively, while the apoptotic index is the highest when the three drugs above is joint together;3. The Ki-67 ratio in xenograft tumors of Taxotere group was significantly reduced than the control group, and Ki-67 ratio in xenograft tumors is lower when Taxotere was joint with Cisplatin or Herceptin respectively, while the Ki-67 ratio in xenograft tumors is the lowest when the three drugs above is joint together.Conclusion 1.Taxotere can obviously inhibit the growth of xenografts of human ovarian cancer SKOV3 cells in nude mice; Down-regulation of Ki-67 in SKOV3 cells,and induction of tumor cell apoptosis might be one of its possible mechanisms;2.There are synergistic mechanism of combinative action of Taxotere and DDP,Herceptin respetively,while the three drugs’ combination can more effectively inhibit the growth of tumor. Related Articles | Metrics

Select

Progress in research for HtrA correlation with preeclampsia 2017, 44 (1):  56-59.  Abstract ( 1194 )   PDF (683KB) ( 6875 )   Preeclampsia，a major cause of maternal mortality in developing world， holds a central public health concern，is a peculiar disease during pregnancy . Studies have shown that preeclampsia is closely related to the cell apoptosis, vasospasm, activation of blood coagulation system, inflammatory reaction and so on. Its pathological change began in the shallow placenta implantation and placental vascular recasting barriers, lead to the placenta tissue ischemia hypoxia, cause endothelial cell damage. HtrA is one of serine protease family, under stress conditions such as cells to heat shock, oxidative stress, inflammation, ischemia-reperfusion and cancer, degradation of misfolded proteins in the cell cytoplasm, participate in the pathological physiological processes such as cell apoptosis, arthritis, embryonic form, nerve degeneration and neuromuscular disorders and cancer. Studies have showen the subtypes are closely associated with preeclampsia. This paper presents the progress of HtrA associated with preeclampsia and its mediated control network, to further understand the pathogenesis of preeclampsia and provide a new treatment method has an important significance. Related Articles | Metrics

Select

Research progress of Thrombelastography in high-risk pregnancy Lei QIAO 2017, 44 (1):  60-63.  Abstract ( 1187 )   PDF (697KB) ( 6953 )   Thrombelastography(TEG) is an image which can reflect the ability of coagulation, blood clotting and fibrinolysis dynamically. TEG can reflect the coagulation state in pregnant women veritably and comprehensively. TEG has important value for predicting the high-risk pregnancy such as recurrent spontaneous abortion, hypertensive disorders in pregnancy. Some peripartum complications such as amniotic fluid embolism, postpartum hemorrhage and venous thrombus embolism can also be evaluated by TEG. Related Articles | Metrics

Select

The Progress for the application security of somatostatin analogues on mother and fetus in pregnancy. 2017, 44 (1):  64-66.  Abstract ( 1036 )   PDF (650KB) ( 6824 )   Somatostatin（SST）is a regulatory peptide, inhibiting the release of many hormones , with a wide range of endocrine inhibitory effect on diseases such as upper gastrointestinal bleeding, acute pancreatitis and acromegaly. The FDA has recognized somatostatin analogues as Class B drugs，and the domestic and foreign clinicians often use them to treat the pregnancy-complicated diseases，but the application security of them in human pregnancy is still inconclusive and worth further studying. Progress for the application security of somatostatin analogues on mother and fetus in pregnancy. Related Articles | Metrics

Select

Progress with aneuploidy prenatal screening in twin pregnancies 2017, 44 (1):  67-70.  Abstract ( 1067 )   PDF (674KB) ( 6917 )   The incidence of aneuploidy in twin pregnancy women is higher than singleton pregnancies women, and the prenatal screening effect is influenced by many factors. Zygosity in twin pregnancy is very important, which directly affects the risk calculation models. Normally, we use ultrasound to detect fetal human chorionic. It can indirectly judge the Zygosity of twin pregnancy. Currently, the fetal chromosomal aneuploidy screening in twin pregnancy is mainly concentrated in: the first trimester nuchal translucency thickness (NT) screening, first trimester combined screening (NT+ serum screening): second trimester serum screening, as well as the noninvasive prenatal testing(NIPT). It involved in many aspects, like the consultation after screening, prenatal diagnosis, dealing in fetal with aneuploidy, ethical and technical. This article gives an overview of the aneuploidy prenatal screening with twin pregnancy. Related Articles | Metrics

Select

Research Progress of Cerebroplacental Ratio Used in Diagnosis of Fetal Intrauterine Anoxia 2017, 44 (1):  71-74.  Abstract ( 1618 )   PDF (687KB) ( 7895 )   Fetal intrauterine anoxia is one of the important causes of perinatal death. Prenatal fetal hypoxia can be effectively predicted, it is great significance to reduce perinatal mortality. Combined detection of fetal doppler blood flow parameters，including the umbilical artery, middle cerebral artery, renal artery, venous catheter, abdominal umbilical vein, reflect the abnormal state of fetal organs, can accurately assess fetal intrauterine hypoxia status, to improve the diagnostic rate of fetal distress. The ratio of cerebral artery and umbilical artery pulsation index was used to eliminate the common interference factors, which ruled out the influence of the base fluctuation, and was affected by the change of blood perfusion impedance. Cerebroplacental rate can reflect the distribution of blood flow of fetal body more than a single vessel, for early evaluation of placenta fetal blood circulation changes, thus a more comprehensive evaluation of intrauterine hypoxia degree, predict adverse outcomes of pregnancy earlier, timely detect the abnormal, timely intervention, improve the perinatal quality, have more clinical value, it is worth to be popularized in clinical application. Related Articles | Metrics

Select

Antibiotics application during the pregnancy and their effects on fetal development Zhide DING 2017, 44 (1):  75-79.  Abstract ( 1186 )   PDF (781KB) ( 6992 )   Antibiotic overuse, which is common phenomenon among all population in China especially pregnant women, can trigger more severe infections by promoting drug-resistant bacteria and also contribute to a long-term harmful effect on embryonic and neonatal development. Although many advantages in antibiotics application in clinics, the underlying huge risks still can’t be ignored. In this article, we review the antibiotics application during pregnancy and its potential side-effects on embryo or newborn development by summarizing the results of retrospective studies and animal experiments presented in published research papers. The former includes the rational appliance of antibiotics in circumstances of artificial rupture of membrane, urinary tract infections, and infections during vaginal delivery; and the latter concludes the newly-reported harmfulness of antibiotics to the embryo or newborn, such as fetal abnormalities, low immunity of newborn to diseases and infant’s obesity subsequently. Thus, the review of the application of antibiotics in clinics during the pregnancy and by-effects on the embryo and newborn development, which can provide theoretical evidences for clinical rational application of antibiotics, is an important caution to our society as well. Related Articles | Metrics

Select

The Association Study Of HLA-G Single Nucleotide Polymorphisms And Severe Preeclampsia 2017, 44 (1):  80-83.  Abstract ( 1052 )   PDF (869KB) ( 6780 )   Human leukocyte antigen (HLA)- G defects can result in the occrence of preeclampsia, in this paper, In order to clarify the role of HLA - G and its single nucleotide polymorphisms(SNPs) in preeclampsia furtherly，we detected the correlation between HLA-G SNPs and preeclampsia in pregnancy women of Chinese Han population in Tianjin. Objective: To detect the distribution of HLA-G SNPs which is one of the susceptibility genes of preeclampsia in severe preeclampsia patients and normal pregnant women, to understand the change of HLA-G SNPs in the patients with severe preeclampsia. Method: The blood samples were collected from 67 cases of severe preeclampsia group and 37 cases of control group, the genomic DNA of samples were extracted, the Exon-2 and Exon-3 of HLA-G SNPs were chosen to evaluate through sequencing, the fluorescent primers were synthesized and PCR was proceeded, the fragments obtained from PCR were sequenced and analyzed to find SNPs, The correlation of SNPs and severe preeclampsia was analyzed by statistical analysis. Results: Two SNPs, C374T and G476A were found in the Exon 2 of HLA-G. Three SNPs, C810T, C859A and G954A were found in Exon 3. There was no significant difference in the distribution of the SNPs between preeclamptic and normal pregnancies. Conclusions：1. Five SNPs (C374T, G476A, C810T, C859A and G954A) were found in the HLA-G Exon 2 and 3, which were not related to severe preeclampsia, these SNPs were synonymous mutations. 2. C374T and G954A were newly discovered HLA-G SNPs which were submitted to the dbSNP and obtained NCBI Assay Id (ss#) 119994701 and 119994702, and the Reference SNP Id (rs#): rs77474325 and rs75783850. Related Articles | Metrics

Select

Clinical analysis of intramural pregnancy 2017, 44 (1):  91-94.  Abstract ( 1051 )   PDF (737KB) ( 6896 )   Objective To identify the clinical features treatment effects risk factors differential diagnosis and treatment of the intramural pregnancy. Methods Retrospective analysis the history of surgery clinical features auxiliary examination diagnosis treatment and pathologic result on 4 patients admitted to our hospital with intramural pregnancy in 5 years. To understand the risk factors differential diagnosis and treatment of the intramural pregnancy. Result Four patients all belong to the mass type of the intramural pregnancy. B-mode MRI diagnoses pregnancy in uterine horn and gestational trophoblastic neoplasia. All of the four patients accept hysteroscopy combined with laparoscopy surgery and supervise HCG drop to the normal level. Conclusions In the clinical doctor should pay attention to the patient of incomplete abortion,HCG can not drop to the normal level or increase,vaginal bleeding and B-mode MRI according to mesometrium mass . Related Articles | Metrics

Select

The Clinical Analysis of 61 Pregnancies with Systemic Lupus Erythematosus Ran-ran DENG 2017, 44 (1):  95-98.  Abstract ( 1141 )   PDF (792KB) ( 6814 )   Objective: To explore the systemic lupus erythematosus with respect to obstetric complications, pregnant outcomes and the manner of delivery. Methods: we reviewed the medical records of SLE pregnant women treated from January 1, 2010 to December 31, 2015 in Tianjin medical university general hospital. According to clinical manifestation, laboratory examination, drug treatment during pregnancy, the patients is divided into the stability of SLE group (35 cases) and SLE activity group (26cases). Results: In terms of pregnancy complications, the incidence of preeclampsia in SLE disease activity group was obviously higher than that in the stability of SLE group（58% vs 0，P=0.000）. The incidence of other complications of premature rupture of membranes, fetal distress, fetal intrauterine growth restriction, postpartum hemorrhage, and oligohydramnios has no statistically significant difference between two groups (P > 0.05). In expect of pregnant outcomes and the manner of delivery, the incidence of prematurity（50% vs 20%，P=0.014）,low birth weight infant（50% vs 20%，P=0.014）, therapeutic abortion（27% vs 3%，P=0.018）in SLE disease activity group was obviously higher than that in the stability of SLE group. However, there has no statistically significant difference between low birth weight infant with preterm birth and low birth weight infant with term birth. The incidence of stillbirth, spontaneous, embryonic development stopping, Neonatal lupus, and cesarean delivery has no statistically significant difference between two groups (P > 0.05). Two patients from four patients with SLE found in pregnancy were diagnosed with lupus in the department of rheumatology clinic due to repeated fetal heart rate fluctuation between 115 ~ 125bpm. Conclusions: Preganat women with SLE are high-risk patients. The incidence of preeclampsia, prematurity, low birth weight infant, therapeutic abortion is high in SLE activity. Pregnancy should be planned after remission for more than six months. Clinical manifestations, blood pressure, and laboratory examination, such as urinary protein, kidney function, immunological indexes, should be closely monitoring during pregnancy. So, we can find SLE disease activity timely and timely treatment in order to reduce the incidence of pregnancy complications and adverse outcomes. It is worth noting that preeclampsia occuring during pregnancy should be be distinguished with lupus and for repeated low heart rate of maternal pregnancy, should also be alert to merge lupus and the repeated slow fetal heart rate during pregnancy should be alert to the lupus. Related Articles | Metrics

Select

The research progress of pathogenesis and clinical applications of endometriosis YANG ZHOU 2017, 44 (1):  103-107.  Abstract ( 1025 )   PDF (737KB) ( 6747 )   Endometriosis is a challenging gynecological disease associated with clinical symptoms such as dysmenorrhea, chronic pelvic pain, menoxenia and infertility. Recent research indicates that EMs can clearly be triggered by genetic aetiology; the combination of dysregulation factors, such as hormones and hormone receptors, inflammation, immunity, adhesion, invasion and angiogenesis, leads to an abnormal pelvic microenvironment and causes the disease. However, due to its complexity, the exact pathogenesis of EMs remains unclear. Nowadays, therapy aiming at key targets can improve the treatment effect but fail to cure the disease. This article summarizes recent research development of pathogenesis of EMs relating to endocrine, inflammation immunity, heredity, angiogenesis, invasion and adhesion. It also reviews new clinical applications of therapy aiming at key targets. Related Articles | Metrics

Select

The linkagesbetween Transforming growth factor β1 and polycystic ovary syndrome 2017, 44 (1):  108-112.  Abstract ( 1009 )   PDF (721KB) ( 6834 )   Polycysticovary syndrome is one kind of endocrine disorder characterizedbyreproductive dysfunction and metabolic abnormalities，but there is no generally accepted conclusion about its etiology and pathogenesis until now. In recent years, much attention has been paid to molecular biology and genetic research concerning PCOS and scholars at home and abroad have found that transforming growth factor beta 1 is closely related to the pathogenesis of polycystic ovary syndrome. In this paper, the linkages of transforming growth factor β1 in the pathogenesis of polycystic ovary syndrome were reviewed in order to provide new ideas and theoretical basis for clinical treatment of polycystic ovary syndrome. Related Articles | Metrics

Select

Isolation and Identification of Stromal Stem Cells from Eutopic Endometrium Functionional layer of Women with Endometriosis Ji-wen ZHANG 2017, 44 (1):  113-117.  Abstract ( 1157 )   PDF (994KB) ( 7129 )   Objective: to get the endometrium stem cells (ESC) for the eutopic endometrial from women with endometriosis (EMs) by using Colony-forming assay and provide the experimental basis for the further study of EMs. Study design: The endometrial samples from women with EMs undergoing fractional curettage respectively. Colony-forming cells (CFCs) were isolated from individual large colony-forming units (CFU) which visible macroscopically and contained >4000 cells. We focused on the difference of CFCs characteristics in women with EMs, including high proliferative potential（MTT assay）, serial clone formation, mammosphere formation of self-renewal, differentiation potential in vitro, and tissue reconstitution in vivo. Result: the optical densities in cell proliferation were more significant higher in the CFCs than N-CFCs ), The CE of CFCs from P1-5 are higher than N-CFCs 。There are statistical significances between the two groups (P<0.05). CFCs originated spheres with a diameter were at least 60μm, spheres were continuously generated at 5-6 day intervals for at least 3 passages with a bigger and bigger size，in contrast, the spheres of N-CFCs did not change much in size after plating for 1 passage, and cannot be passaged serial .The protein of CFCs was negative for CK-19 before induction culture, but were expressed at CFCs, which cultured with the induction medium for 21 days. The endometriotic lesion-forming efficiency (100%) in the CFCs group was higher than that of N-CFCs group(40.63%), when CFCs and N-CFCs were inoculated into the abdominal subcutaneous of nude mice, respectively. Reconstruction of endometriotic lesions in the CFCs group with an average volume at (30.95 ±12.13)mm3 was bigger than the N-CFCs group (19.12±10.98)mm3. Conclusion: There are stem cells (SC) in the function layer of endometrium in EMs patients and it can be isolated from eutopic endometrium using colony-forming assays. The study has given a simple method to get ESC easily for researching EMs. References | Related Articles | Metrics

Select

Association between anti-Mullerian hormone and insulin resistance in patients with polycystic ovary syndrome 周 伟伟 2017, 44 (1):  118-120.  Abstract ( 886 )   PDF (746KB) ( 6708 )   【Abstract】Objective: To study relationship of the anti-Mullerian hormone (AMH) and and insulin resistance (IR) in non-obese women with polycystic ovary syndrome (PCOS). Methods: The study enrolled 102 non-obese women with PCOS, in which insulin resistance group (PCOS with IR, n = 48), non-insulin-resistant group (PCOS without IR, n = 54), and the age and body mass index matched 50 non-obese healthy people as a control group. the three groups of c and the relationship between AMH and PCOS with IR. Results: Serum AMH levels and HOMA-IR in PCOS with IR group were significantly higher than PCOS with IR and control group, (p <0.05). Conclusion: Elevated serum AMH levels were found in non-obese PCOS with IR patients, AMH may be associated with PCOS with IR. 【Key words】Polycystic ovary syndrome, anti-Mullerian hormone, insulin resistance Related Articles | Metrics