Table of Content

15 December 2013, Volume 40 Issue 6
Previous Issue    Next Issue
---
述评
专家论坛
综述
论著
综述
论著


For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

述评

Select

Management of Early Multiple Pregnancy LIN Qi-de;MA Ning 2013, 40 (6):  483-484.  Abstract ( 1345 )   PDF (413KB) ( 4796 )   Multiple pregnancy is considered of high risks for mother and babies .Women with multiple pregnancy have an increased risk of miscarriage,anaemia,hypertensive disorders, haemorrage, operative delivery, and postnatal illness.Fetal risks of stillbirth, preterm birth, neonatal mortality and morididy are considerable higher in multiple birth than in singleton birth.Therefore, management of early multiple pregnancy including regular prenatal care, with special emphasis on early diagnosis of twin-twin transfusion syndrome, Down syndrome and preterm birth is very important. Related Articles | Metrics

Select

The Development and Controversy of Diagnosis and Management to Gestational Diabetes Mellitus YANG Hui-xia;ZHU Wei-wei 2013, 40 (6):  485-488.  Abstract ( 1169 )   PDF (591KB) ( 4787 )   The diagnostic criteria of gestational diabetes mellitus has being changed continuously. Based on the hyperglycemia and adverse pregnancy outcome study, most international academic organizations including World Health Orgnization and Ministry Heath of China published new criteria, while there are still some arguments. After introduced the development of the diagnostic criteria and risk factors, the article presented the study of metformin in gestational diabetes the offspring followup and proposed to adopt comprehensive intervention to face the challenges to our clinical management in China. Related Articles | Metrics

专家论坛

Select

Prediction of Preeclampsia in the First Trimester CHEN Xu;CHANG Ying 2013, 40 (6):  489-491.  Abstract ( 1259 )   PDF (392KB) ( 4760 )   Preeclampsia is a obstetrical complication with high perinatal mortality and morbidity. It is characterized by shallow trophoblast invasion in maternal uterine spiral arteries. Despite there are more and more understanding on the pathophysiology of preeclampsia，it is difficult to predict effectively in time in clinic. There are some relationship between the disordered placenta function and the poor pregnancy outcomes. However, any single parameter of placenta function could not be used as a exact predictor for preeclampsia. The improved ultrasound will provide a useful novel tool for prediction，but measurement of ultrasound need to be standardized. The test of biochemical markers in the first trimester, combined with the uterine artery Doppler screening, may be a promising screening tool. Related Articles | Metrics

Select

Prediction of Mater-fetal Complications and Standardized Management of Multiple Pregnancy in the Early Term QU Xiao-xian;YING Hao 2013, 40 (6):  492-495.  Abstract ( 1320 )   PDF (596KB) ( 4762 )   With the progress of assisted reproductive technology and the increase of women′s reproductive age，the incidence of multiple pregnancy is also increased. Multiple pregnancy increased the morbidity and mortality of maternal-fetal complications. Therefore，prediction of mater-fetal complications and the standardized management of multiple pregnancy in the early term helps to reduce the maternal-fetal risks including abortion，anemia，preeclampsia and gestational diabetes mellitus. According to recent studies，the management of multiple pregnancy in the early term should pay attention to chorionic judgment，screening of fetal complications（screening for aneuploidies and ultrasound scanning）， prediction of mater complications and standardized monitoring during early pregnancy,thereby reducing the mater-fetal complications and improve mother and child outcome. Related Articles | Metrics

综述

Select

Recent Advances in Retinol Binding Protein-4 and Gestational Diabetes Mellitus ZHOU Ping;CHEN Dan-qing 2013, 40 (6):  496-499.  Abstract ( 1345 )   PDF (489KB) ( 4733 )   Retinol binding protein-4 （RBP4） is a newly discovered adipocytokine mainly secreted by liver and fat cells，which can regulate glucose and lipid metabolism. RBP4 is closely related to insulin resistance and metabolic syndrome. Gestational diabetes mellitus （GDM） is a common pregnancy complications，its incidence is increasing，but its pathogenesis is unclear，which is considered to be associated with insulin resistance，regardless of compensatory insulin secretion. Recent studies on adipokines including RBP4 have suggested that RBP4 is not only the association factor in obesity and diabetes mellitus and insulin resistance，but also the novel factor closely related to the pathogenesis，prognosis and risk of type-2 diabetes mellitus and metabolic syndrome in the women with previous GDM and offspring of GDM. However，RBP4 is still a controversial adipokine. Related Articles | Metrics

Select

Progress about Immunogenetics of Gestational Diabetes Mellitus LYU Ya-su;ZUO Chang-ting 2013, 40 (6):  500-503.  Abstract ( 1244 )   PDF (787KB) ( 4736 )   Gestational diabetes Mellitus（GDM） is a clinical syndrome with abnormal expression of multiple genes and environmental factors，its incidence increased year by year，so explore the pathogenesis and pathophysiology of GDM is very important for the prediction and intervention. Pregnancy related insulin resistance exceeds the limits of compensatory β islet cell′s function is a recognized reason of GDM, however, the exact pathogenesis is not fully understood. In recent years, new studies have found that the immune and genetic factors play important roles in the occurrence and development of GDM. This article is from the view of immune and genetic，discuss the T cells, HLA complexes and other changes in patients with gestational diabetes，wishing to provide new ideas for further research of GDM′s pathogenesis. Related Articles | Metrics

Select

Research Progress in Risk Factors of Gestational Diabetes Mellitus LI Bao-juan;DONG Ling 2013, 40 (6):  504-506.  Abstract ( 1314 )   PDF (432KB) ( 4707 )   Gestational diabetes mellitus (GDM) is one of common complications during pregnancy, maternal and child safety threat, increasing the risk of pregnancy. With the increasing incidence of GDM, people pay more and more attention to the researches on its risk factors. Gestational insulin resistance is now more accepted as GDM′s main pathogenesis. In addition to the pregnant women of traditional factors such as age, race, family history of diabetes, pregnant women before pregnancy，and the body mass index （BMI），pregnant women in vivo storage content genetic polymorphism and infectious factors are associated with the occurrence of GDM. The papers in recent years on the risk factors of GDM progress are reviewed in the paper. Related Articles | Metrics

论著

Select

The Effect of Combined-epidural Labor Analgesia on Stages of Labor Intrapartum Endocrine and Neonate Blood Sugar in Gestational Diabetes Mellitus JIANG Yan-min;HE Ping;LIU Hui-shu;LAI Yu-mian;QIN Shuang 2013, 40 (6):  507-509.  Abstract ( 1092 )   PDF (488KB) ( 4757 )   Objective：To evaluate the effect of combined-epidural labor analgesia（CSEA） on stages of labor intrapartum blood sugar， serum cortisol and neonate blood sugar in women with gestational diabetes mellitus（GDM）. Methods：48 cases of primiparas diagnosed GDM received CSEA， and were observed as studied group and the other 45 cases without receiving CSEA were observed as control group. All the stages of labor ，intrapartum blood sugar， serum cortisol， cortisol in umbilical cord blood and neonate blood sugar were determined. Results：①The blood sugar in studied group were significantly lower than the control in the first、 second and third hours during active phase（P＜0.05） and the blood sugar in studied group is ralatively stable . The serum cortisol of the pregnant in studied group were significantly lower than the control in end of the active phase（P＜0.05）. ②The cortisol in umbilical cord blood in studied group were significantly lower than the control（P＜0.05）. The blood sugar of neonate in studied group were significantly higher than the control（P＜0.05）. ③The time of the active phase in studied group were significantly shorter than the control（P＜0.05）. Conclusions：CSEA maybe could shorten the time of the active phase and effectively eliminate the fluctuation of blood sugar and serum cortisol in gestational diabetes mellitus， and also improve blood sugar of neonate. Related Articles | Metrics

Select

The Levels of Visfatin，Lipid Metabolism and Insulin Resistance and Their Relationship in Women with Preeclampsia WU Jing-jing;YAN Jian-ying 2013, 40 (6):  510-513.  Abstract ( 1240 )   PDF (577KB) ( 4775 )   Objective：The purpose of this study was to detect the serum levels of visfatin，lipid metabolism and insulin resistance in preeclampsia（PE），and to discuss their roles in the pathogenesis of PE. Methods：A total of 96 women with PE and 96 normal pregnant women as control participated in this study. All cases are admitted to the Second Affiliated Hospital for delivery from July 2010 to January 2011. Patients with PE were divided into severe PE group（n=61） and mild PE group（n=35）. Enzyme-linked immunosorbent assay（ELISA） was used to detect the levels of visfatin. Radioimmunoassay assay was used to detect the levels of fasting insulin. Homeostasis model assessment insulin resistance index（HOMA-IR）. Furthermore，clinical and biochemical parameters were recorded，such as body mass index（BMI），systolic blood pressure（SBP），diastolic blood pressure（DBP），and to conduct the correlation analysis. Results：①The level of serum visfatin in PE group significantly elevated than that in control group，the level in severe PE group significantly elevated than that in mild PE group（P＜0.05）. ② In PE groups，the serum visfatin were positively related to the serum TG，TC，LDL，ApoB，FINS and HOMA-IR，while the serum visfatin was negatively related to the serum HDL（P＜0.05）. ③In PE groups，the serum levels of visfatin were positively related to the serum TG，SBP，DBP，and HOMA-IR（P＜0.01）. Conclusions：Elevated visfatin expression in serum may be associated with the pathogenesis and development of preeclampsia. Dyslipidemia and insulin resistance may be associated with elevated visfatin expression in patients with PE，and may be related to participation in the pathogenesis of preeclampsia. Related Articles | Metrics

Select

Expression of Catechol-O-Methyltransferase in Placenta and Its Relationship with Preeclampsia LAI Bao-ling;WANG Chen-hong;WANG Jie;WU Hui-fen;ZHOU Lu 2013, 40 (6):  514-516.  Abstract ( 1222 )   PDF (509KB) ( 4676 )   Objective：To investigate the expression of catechol-O-methyl- transferase（COMT） in placenta from patients with preeclampsia and the relationship between COMT and the pathogenesis of preeclampsia. Methods：15 patients with preeclampsia and 15 normal pregnant women were enrolled. Clinical characteristics and placental tissue were collected. placental COMT expression was determined by quantitative real-time PCR technique. Results：The level of COMT mRNA in Placenta from the preeclampsia group was significantly decreased than that in the normal pregnant women（P＜0.001）．Adjusted by age and body mass index（BMI），the COMT expression was negatively correlated with serum uric acid，24-hour urine protein and mean artery pressure（all P＜0.001）. Conclusions：The decreased expression of COMT in placenta from preeclamptic patients suggests that COMT may be involved in the pathogenesis of preeclampsia. Related Articles | Metrics

Select

aternal Serum Leptin，β-hCG ADAM12 Levels between Second and Third Trimester of Pregnancies and Prediction of Preeclampsia YAN Jian-ying;LIN Yuan;ZHANG Dong-mei;CUI Xiao-mei;LIU Qing-min;CHEN Su-qing;HUANG Xiao-yan;SONG Jian-rong;XUE Hui-feng 2013, 40 (6):  517-520.  Abstract ( 1309 )   PDF (668KB) ( 4772 )   Objective：To examine maternal serum concentrations of leptin，beta-human chorionic gonadotropin（β-hCG） and ADAM12 between second and third trimester of pregnancy， and investigate whether an appropriate combination of those markers may be an effective means of screening for preeclampsia（PE）. Methods：Samples from 189 pregnant women who presented to Fujian Maternity and Child Health Hospital for prenatal care from June 2007 to May 2008 were included in this prospective study，of which 25 were subsequently developed PE as case group and 164 were normal pregnant women as control group. The maternal serum was conducted between their second（between 20th to 24th week of pregnancy） and third（between 30th to 34th week of pregnancy） trimester of pregnancy. The cutoff point was determined using a receiver operating characteristic curve. Results：①The second trimester level of serum β-hCG and ADAM12 in case group were significantly higher than normal group（P＜0.001）. ②The third trimester level of serum leptin，β-hCG and ADAM12 in case group were significantly higher than normal group （P＜0.001）. ③ The third trimester level of serum leptin，β-hCG and ADAM12 in case group were significantly higher than their second trimester level（P＜0.05）. The third trimester level of serum leptin in normal group was significantly higher than their second trimester level （P＜0.001）. ④The cutoff concentration of β-hCG was 32 μg/L and ADAM12 was 818 μg/L in second trimester of pregnancies. Combining the values of β-hCG and ADAM12 to form a screening test would detect an estimated 82.61% of affected pregnancies. There was no statistically significant difference between combining β-hCG and ADAM12 AUC and single β-hCG or ADAM12 AUC（P＞0.05）.⑤The cutoff concentration of leptin was 23 μg/L， β-hCG was 37 μg/L and ADAM12 was 900 μg/L in the third trimester of pregnancies. Combining the values of leptin，β-hCG and ADAM12 to form a screening test would detect an estimated 92.31% of affected pregnancies. AUC of combined leptin，β-hCG and ADAM12 is greater than the single leptin or β-hCG leptin（P＜0.05）, but there was no statistically significant difference between combined leptin，β-hCG and ADAM12 and single ADAM12 （P＜0.05）.⑥Combining the second and third trimester levels of serum β-hCG to form a screening test would detect an estimated 81.25% of affected pregnancies. Combining the second and third trimester levels of maternal serum ADAM12 to form a screening test would detect an estimated 90.48% of affected pregnancies. Conclusions：This study shows that measuring levels of β-hCG and ADAM12 during the second and third trimester of pregnancy are useful in clinical practice to identify pregnant women who will develop preeclampsia. Related Articles | Metrics

综述

Select

The Role of Transmembrane 4 Superfamily Members in the Embryonic Implantation LIANG Ying-qiu;GAO Tian-yang 2013, 40 (6):  521-524.  Abstract ( 1212 )   PDF (602KB) ( 4748 )   The implantation of the blastocyst into the maternal uterus is a crucial step in mammalian reproduction. The successful establishment of pregnancy requires an interaction between the blastocyst and receptive uterus，then trophoblasts invade the endometrium accurately. And the decidualization of endometrium is considered a major factor in this process. Similar to tumor metastasis，embryonic implantation also include identification，adhesion，invasion and transfer process. Differently，the invasion of trophoblast has a “self-limited” cell behavior，and it is exactly controled by a complex network of various cells and molecules. Transmembrane 4 superfamily members play an important role in a series of biological actions，such as cell adhesion，invasion and transfer，not only in the tumor metastasis，but also in the embryonic implantation. Related Articles | Metrics

Select

The Role of CD4+T Cells in Unexplained Recurrent Spontaneous Abortion：an Update WANG Wen-juan;LIN Qi-de 2013, 40 (6):  525-529.  Abstract ( 1407 )   PDF (695KB) ( 4707 )   The human gestation is a semi-allograft and hence antigenically foreign to the mother. Therefore, the process of implantation may include mechanisms to prevent allograft rejection, but once the immunological tolerance becomes imbalanced, pathological pregnancy, such as spontaneous abortion could occur. Unexplained recurrent spontaneous abortion （ＵＲＳＡ） is thought to be caused by immunological rejection of the fetus by the mother. CD4+ T cells which include Th1, Th2, regulatory T cells （Ｔｒｅｇ） ａｎd Th17 cells, play important roles in allograft rejection. Maternal peripheral blood and decidual CD4+ T cells augmentation have been implicated in URSA. Mechanisms of immune tolerance is in the deepening，from the Th1/Th2 immune balance to the negative regulation of Treg cells. Recently, the Th17/Treg balance, has been described to paly an important role in the immunological rejection. In this paper the role of CD4+ T cells in URSA is reviewed. Related Articles | Metrics

Select

Maternal Decidual Microenvironment and Trophoblast Cell Invasion ZHU Xiao-ming;HAN Tao 2013, 40 (6):  530-533.  Abstract ( 1353 )   PDF (580KB) ( 4718 )   During normal pregnancy，placental trophoblast cells have the ability to invade-like tumor cells. However，unlike tumor invasion，trophoblast cell invasion to the maternal deciduas is under highly control and is a normal physiological behavior. The overinvasion and inadequate invasion can lead to such pathologies of pregnancy as choriocarcinoma，miscarriage and preeclampsia，respectively. Nowadays，the mechanisms responsible for trophoblast cell invasion to maternal deciduas remain unidentified. Evidence indicates that the maternal decidual microenvironment exerts important influence over trophoblast cell invasion. The further study will be helpful to understand the pathogenesis of pathological pregnancy. Related Articles | Metrics

Select

Toll-like Receptors on Mechanism of Disease in Pregnancy DENG Li-zhe;SHAO Yong 2013, 40 (6):  534-536.  Abstract ( 1253 )   PDF (401KB) ( 4733 )   Toll-like receptors（TLR） is a major concern pathogen recognition receptors nowadays. It plays a role in innate immunity via the identification of the pathogen associated molecular patterns. It connects innate immunity and acquired immunity by stimulating the signaling cascade of reactions lead to the production of cytokines and expression of costimulatory factors. Maternal-fetal trophoblast cells and amniotic membrane of cells specific to the interface，and so on，by TLR，also can identify the pathogen and involve in the innate immune response，which in turn have an impact on pregnancy outcome. Related Articles | Metrics

Select

Environment Factors and Birth Defects GUO Xiao-han;HE Jing 2013, 40 (6):  537-540.  Abstract ( 1243 )   PDF (652KB) ( 4673 )   Birth defect is one of the main causes of the death of perinatal infant，and it is also supposed to be the major cause that leads to stillbirth，deadbirth and affect the growth of neonatals. It relates to environment factors，genetic factors，or the interaction of each other. For recent years，more and more researches have focused on the environment risk factors leading to birth defects. It was found that，birth defects were related to harmful physical or chemical factors(such as radiation,hyperthermia，lead，cadmium，zinc and folic acid)，maternal active and passive smoking， exposure to organic solvents and environmental pollution or exposure to agricultural toxic，drinking water disinfection by-products and so on. This paper aims to investigate the relationship between environment and birth defects，to provide a theoretical basis for reducing birth defects. Related Articles | Metrics

Select

Special Ectopic Pregnancy： An Analysis and Recent Progresses LIU Chun-xian;ZHU Ying-jun 2013, 40 (6):  541-544.  Abstract ( 1100 )   PDF (792KB) ( 4708 )   Ectopic pregnancy is a common gynecologic acute abdominal disease， and along with the increase of gynecological inflammation，sexual transmitted disease，uterine cavity operation and caesarean section，the incidence of special ectopic pregnancy(SEP) is increasing over past several years. SEP includes caesarean scar pregnancy，ovarian pregnancy，rudimentary uterine horn pregnancy，cervical pregnancy and abdominal pregnancy. Some ectopic pregnancies are especially difficult for early diagnosis and have the risk of mis diagnosis，thus becoming life-threatening，because they have generally few apparent clinical features and signs which，if any，are not typical. With the application of ultrasonic skills within the diagnosis of pregnancy and the widely acceptance of the awareness of bearing and rearing better children，the threats of ectopic pregnancy is reducing year by year. The three major clinical features are menopause，abdominal pain，and vaginal bleeding，and different sort of SEP has its typical features each. It is an urgent issue for gynecological surgeons and emergency department of general hospital that the typical features of SEP，early diagnosis and timely treatment be emphasized. Related Articles | Metrics

Select

Role of TLR3 in Cervical Antiviral Immunity and Cervical Lesions QU Quan-xin;MI Ruo-ran 2013, 40 (6):  545-547.  Abstract ( 1145 )   PDF (401KB) ( 4652 )   Toll-like receptor 3（TLR3） is an important member of the family of pattern recognition receptors（PRRs） in innate immunity and can be specific to identify the virus double-stranded RNA，activate the protective response and clean viruses. TLR3 expresses mainly in immune cells and also in epithelial cells and epithelial carcinomas. TLR3 expression is associated with infection，inflammation and tumor progression. Female genital tract mucous membrane is an important barrier against pathogenic microorganism infection，TLR3 expression is associated with genital tract physiology and pathology. The review will discuss the role of TLR3 in cervical HPV persistent infection，intraepithelial neoplasia and cervical cancer. Related Articles | Metrics

Select

Research Progress of the Progesterone Resistance Mechanisms of Endometriosis HI Xiu;XU Wei;DAI Hui-hua;WANG Xiu-li 2013, 40 (6):  548-550.  Abstract ( 1308 )   PDF (599KB) ( 4762 )   Endometriosis（EMs） is defined by the presence of visible endometrial implants outside the uterus，mostly occurred in the pelvic organs. The cyclical bleeding of ectopic endometrial lesions leads to serious pelvic pain and infertility of women. EMs is widely viewed as an estrogen-dependent disorder. Progesterone is the classic drug to antagonist estrogen. But in the clinical practice，the majority of the endometriosis patients show low reaction or non-reaction on progestin therapy. Attenuation of progesterone response at the level of the endometrium may be expected to have a deleterious impact on endometrial receptivity and fertility. These phenomena are called progesterone resistance of EMs. The progesterone resistance mechanism involves the defect of the progesterone receptor（PR），the change of progesterone receptor co-regulators and the genetic and environmental factors as well as ERα/ERβ. The aim of this review is to highlight recent findings regarding the progesterone resistance mechanisms and to provide theoretical basis for overcoming progesterone resistance. Related Articles | Metrics

Select

Research Progress of Targeted Ultrasound Microbubble in Ovarian Cance ZHANG Jin-yi;ZHU Yuan-fang 2013, 40 (6):  551-554.  Abstract ( 1200 )   PDF (604KB) ( 4720 )   Ovarian cancer is the leading cause of mortality in the gynecologic malignant tumor. 80% of patients are already in the middle or late stage of ovarian cancer when presenting with symptoms. Qualitative and location diagnosis in the early stages of the cancer is one of the key factors to improve the survival rate of patients. Nano-scale contrast agent has the characteristic of the strong blood vessels penetrating ability and confocal imaging. Tumor-specific targeting microbubbles could be generated by conjugating tumor-specific antibodies or ligands into the surface of the microbubbles which can results in selectively accumulation, long resident time in cancer tissue, so it can generate a significantly enhanced signal in the target area. Tumor-specific targeting microbubbles entrapped therapeutic genes or drug could be destroyed by ultrasonic wave and released genes or drug in the target site, which results in improving treatment effect. Related Articles | Metrics

论著

Select

The Study on Human Compacted Embryo and Blastocyst Transfer SHE Hong;HU Yan-qiu;LI Xiao-cong 2013, 40 (6):  555-557.  Abstract ( 1503 )   PDF (446KB) ( 4704 )   Objective：To explore the posibility of single human compacted embryo and blastocyst transfer. Methods：There were 231 cycles of human frozen-thawed embryo transfer （FET） in the Center of Reproductive Medicine of Northern Jiangsu People′s Hospital. The study compared embryos implantation rate，clinical pregnancy rate and multiple pregnancy rate among cleavage-stage embryo group，compacted embryo group and blastocyst group of human frozen-thawed embryo. Results：The number of embryo transfer is significantly lower in the compacted embryo group compared to the cleavage-stage embryo group [（1.35±0.50) vs.（1.73±0.59），t=-4.902，P＜0.001）]，the embryos implantation rate and clinical pregnancy rate of compacted embryo group are higher than cleavage-stage embryo group （49.58% vs. 23.00%，χ2=24.567，P＜0.001；53.41% vs. 34.15%，χ2=7.805，P=0.005）. The embryos implantation rate of blastocyst group is higher than cleavage-stage embryo group （46.88% vs. 23.00%，χ2=8.214，P=0.004）. There was no statistics difference on the multiple pregnancy rate among the three groups（χ2=2.231，P=0.328）. Conclusions：Compacted embryo or blastocyst transfer causes a satisfactory embryo implantation rate and clinical pregnancy rate per transfer cycle，and minimizes the multiple pregnancy rate at the same time. They are very useful for single embryo transfer. Related Articles | Metrics

Select

Urethral Sphincter Injury and Repair of Mechano Growth Factor mRNA Expression in the Female Rats WANG Juan;WANG Zhi-lian;HAO Min 2013, 40 (6):  558-560.  Abstract ( 1140 )   PDF (458KB) ( 4692 )   Objective：To observe the expression situation of the partial endocardial mechano growth factor mRNA in the recovery process of the injured female WISTAR rats′ urethra sphincter who have childless show whether the machinery growth factor does participate in muscle cell regeneration and recovery process of Wistar rats′ urethra sphincter. Methods：Divide 60 female Wistar rats who have childless into two groups stochastically. In the experimental group，there are 30 of them whose urethra sphincters are injured like birth injury by obstetrician′s instruments. And then execute them and take out the urethra on the 1sｔ，４ｔｈ，7th，10th and14th day respectively（6/d）. There are 30 as the blank control group，Execute them and take out the urethra，too. On all specimens，the comparative quantitation PCR of internal reference should be carried on，then examine the expression situation of the partial mechano growth factor. Results：In the blank control group，the partial endocardial machinery growth factor mRNA only has extremely little expression quantity，but the expression of it increases significantly on the 1st day after the urethra sphincter was injured，and increases gradually，then reaches the peak on the 7th day,and then drops gradually. However，the expression quantity on the 14th day is still higher than that in the blank control group（P＜0.05）. Conclusions：The expression quantity of the partial endocardial machinery growth factor mRNA increases in the recovery process of the injured female Wistar rats′ urethra sphincter who have childless，and the expression quantity is different in different injured time. It shows that the machinery growth factor does participate in muscle cell regeneration and recovery process of Wistar rats′ urethra sphincter. Related Articles | Metrics

Select

Effects of Autophagy on Cisplatin Induced Cervical Cancer Hela Cells Death in Vitro WANG Zan-hong;ZHANG Jing;ZHANG Na;BAI Tao 2013, 40 (6):  561-564.  Abstract ( 1241 )   PDF (1904KB) ( 4662 )   Objective：To investigate the effects of autophagy on cisplatin-induced cervical cancer Hela cell death，and observe impact of Beclin 1 on sensitivity of Hela cells to cisplatin in vitro. Methods：Hela cells were treated with cisplatin in different concentration， and MDC dyeing was explored to measure the formation of autophagic vacuoles and Western blot was used for detecting protein expression of Beclin 1 and LC3. The eukaryotic expression vector of Beclin 1 was transfected via lipofectamine into Hela cells. MTT assay was employed to observe the effect on the IC50 value of cisplatin，and flow cytometry was employed to observe the percentage of autophagic cells and apoptotic cells. Results：The autophagy vesicles were increased and the expression of Beclin 1 and LC3 were significantly improved in a dose-dependent manner in Hela cells treated with cisplatin（P＜0.05）. The eukaryotic expression vector pcDNA3.1（+）-Beclin1 significantly improved the expression of Beclin1 in Hela cells（P＜0.05）. MTT assay showed the IC50 value of cisplatin decreased from 1.0 mg/L to 0.5 mg/L（P＜0.05），and more autophagic cells and apoptotic cells were identified in pcDNA3.1（+）-Beclin1 group than the other groups. Conclusions：Autophagy play an important role in the process of cisplatin-induced Hela cells death，and Beclin 1 overexpression can increase the sensitivity of Hela cells to cisplatin. Related Articles | Metrics

Select

The Significance and Successful Construction of Stable Transfection of Hela Cells with Lentiviral Vector LIU Long-yang;ZHANG Di-kai;YI Juan-juan;CHEN Qing;ZHANG Bing-zhong;CHEN Zhi-liao;LIANG Jin-xiao;LIN Zhong-qiu 2013, 40 (6):  565-567.  Abstract ( 1507 )   PDF (1705KB) ( 4781 )   Objective：To construct the stable transfection Hela cell lines with lentiviral vector which may interfer the expression of ALDH-1，and to further explore the biological characteristics between the expression of ALDH-1 and Hela cell lines. Methods：Hela cells were cultured in vitro，added the different concentration of puromycin to screen，，and to determine the best screening concentratioｎ；Hela cells were tested from 24-well plates，threw away the old culture medium， and added 400 μL of new medium to the plates，and then added four kinds of lentivirus solution（ALDH-1， ALDH1-1719， ALDH1-740， ALDH1-921，and there was a kind of lentivirus which has the best interfering effect） and one kind of negative control lentivirus solution （LV3-NC） to the plates， at last added Polybrene liquid to them. Every lentivirus solution volume was added about 50 μL （each of viral titers were 1×108 TU/mL），and the Polybrene was added about 0.5 μL （concentration of 5 mg/L） respectively. Fresh culture medium was changed after 24 hours，and observe the fluorescence after 72 hours. At last，using the best screening concentration of puromycin to screen the transfection Hela cells，and to get the stable transfection cell lines. Results：The best screening concentration of puromycin was 0.9 mg/L，lentiviral vectors were successfully transfected into Hela cells，and the stable transfected cell lines were successfully obtained after two weeks. Conclusions：Hela cells which has stable interference of ALDH-1 were successfully constructed. Related Articles | Metrics

Select

Analysis of Perception，Attitude and Utilization of Oral Contraceptives among Population with History of Breast Cancer and Ovarian Cancer LI Hong 2013, 40 (6):  568-570.  Abstract ( 1388 )   PDF (446KB) ( 4677 )   Objective：To investigate the perception，attitude，and utilization of oral contraceptives(OC) among population with history of breast cancer and ovarian cancer. Methods：All of 92 females who had history of breast cancer and ovarian cancer among first level relatives were surveyed on perception，attitude，behavior，and information need. Results：There were 65.2% of participants used OC，and 40.6% of them reported that history of cancer was a major reason for not using OC. Most of participants wanted to know more information about OC and preferred to get information through health education handbook. Conclusions：Among females with history of breast cancer and ovarian cancer，it was helpful to choose ideal contraceptive methods when they had a good perception on OC. Related Articles | Metrics

Select

Influence of Misoprostol Combined with Oxytocin on the Serum Levels of AngiotensinⅡ and Coagulation Factors after Parturient Postpartum ZHONG Jin-pen;HE Yan-hui;ZHU Xiao-chun;WANG Hong-bo;WANG Quan-zhen 2013, 40 (6):  571-573.  Abstract ( 1231 )   PDF (423KB) ( 4656 )   Objective：To investigate the influence of misoprostol combined with oxytocin on serum levels of angiotensin Ⅱ (AngⅡ) and coagulation factors after parturient postpartum，which is a useful reference for effective treatment of postpartum hemorrhage. Methods：120 parturients with postpartum hemorrhage from our hospital during Dec 2010 to Jan 2013 were included in this study. Those high-risk factors were pregnancy hypertension，placental abruption，placenta previa, fetal macrosomia and multiple pregnancy. Those cases were randomly divided into three groups，each with 40 cases. Parturients in group A were immediately administrated with misoprostol 400 μg through rectum after baby delivery. Parturients in group B were immediately administrated with oxytocin 20 U through palace intramuscular，at the same time，with oxytocin 20 U through intravenous infusion. Parturients in group C were immediately administrated with oxytocin 20 U through palace intramuscular and misoprostol 400 μg through rectum. The postoperative bleeding volume was measured by volum and weight after postpartum 2 h and 24 h. The serum levels of angiotensin and coagulation factors were measured by ELISA. The systolic pressures before childbirth and post partum were observed. Results：There were not significant differences in the serum levels of AngⅡbetween antepartum and postpartum, in both group A and group B（P＞0.05） while this difference was significant in group C（t=7.196，P=0.009）. The same status was found in the serum levels of two coagulation factors in three groups. The hemorrhage volumes post partum 2h, 24h of group A and group B were significantly increased than that of group C （P＜0.05）. The differences of those systolic pressures before childbirth and post partum 2h, in three groups, were not significant （P＞0.05）. Conclusions：Misoprostol combined with oxytocin can significantly increased serum levels of angiotensin and coagulation factors when to treat effectively postpartum hemorrhage. Related Articles | Metrics