Table of Content

15 December 2014, Volume 41 Issue 6
Previous Issue    Next Issue
---
述评
综述
论著


For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

述评

Select

The Indication and Principle of Management for Patients with Ovarian Malignancies Undergoing Fertility-sparing Surgery GAO Yong-liang 2014, 41 (6):  581-583.  Abstract ( 1152 )   PDF (392KB) ( 4848 )   Young patients with ovarian malignancies undergoing fertility-sparing surgery(FSS) should be limited to early stage(Ⅰa) with favorable histology, such as high or moderate differentiation, non-clear-cell histology. But for those with advanced stage or unfavorable histology, generally FSS is not recommended. In patients with borderline ovarian tumor, their age is relatively young and most with early stage, after FSS even with ovariocystectomy, the prognosis is not affected. Their fertility outcome is good. In patient with germ cell tumor deal with FSS, most literature reports are dysgerminoma. But for other types of germ cell tumors, or advanced stages, usually FSS is not recommended unless the patient and her family have a strong will. The patients undergoing FSS due to the specific situation is different, the operation should vary from person to person, and individualized. Related Articles | Metrics

综述

Select

Susceptibility Genes of Hereditary Ovarian Cancer Syndrome ZHU Jue;ZHANG Ke;PAN Lu;MA Sheng-lin;QIAN Jian-hua 2014, 41 (6):  584-587.  Abstract ( 1147 )   PDF (561KB) ( 4887 )   Ovarian cancer is one of the three common gynecological malignancy with a highest mortality rate. Due to the absence of specific symptoms and the lack of sensitive screening for the early detection of ovarian cancer，the majority of patients（60%-65%） had advanced stage at diagnosis. In ovarian cancer，hereditary ovarian cancer syndrome（HOCS） occupy a certain proportion. HOCS often associated with the occurrence of mutations in susceptibility genes. There are at least 16 genes implicated in HOCS to date. HOCS was attributed mostly to mutations in BRCA1 and BRCA2. Recently，three new ovarian cancer susceptibility genes have been identified：RAD51C，RAD51D，and BRIP1. Detection of related susc eptibility genes may contributes to the early diagnosis of ovarian cancer risk groups. This article is about the related susceptibility genes of HOCS. Related Articles | Metrics

Select

Role of Tumor Stroma in Ovarian Cancer MA Xiao-li;WANG Jian-dong;DUAN Hua 2014, 41 (6):  588-590.  Abstract ( 1128 )   PDF (394KB) ( 4786 )   The tumor stroma consists of stromal myofibroblasts，endothelial cells，and leukocytes. A large amount of evidence indicates that tumor stroma could produce proteases，growth factors，immune response-modulating proteins and so on and respond to stimuli initiated in the tumor cells to establish a bi-directional communication network in the microenvironment to promote tumor cell proliferation，apoptosis，cell adhesion and tumor invasion. They are closely related to tumor growth and progression. Recently，studies indicate that tumor stroma played an important role in the development and invasion and metastasis of ovarian cancer，and is related to clinicopathological characteristics and prognosis of ovarian cancer. Tumor stroma has been regarded as a hot target for biological intervention in treatment of ovarian cancer. Related Articles | Metrics

Select

Progress of Ovarian Cancer Stem Cells and Epithelial-mesenchymal Transition YAO Xiao-fen;LAI Dong-mei 2014, 41 (6):  591-595.  Abstract ( 1340 )   PDF (670KB) ( 4836 )   Cancer as a disease driven by cancer stem cells is a concept that has emerged over the last few years. Along with varieties of cancer stem cells（CSCs） have been identified，the isolation and identification of ovarian cancer stem cells（OCSCs） developes quickly. OCSCs is a self-renewal and differentiation of cells. Recently，increasing evidences suggest that CSCs are potential candidates for mediating metastatic progression and chemoresistance. Studies have also indicated the process of epithelial-mesenchymal transition（EMT） plays an important role in regulating cancer metastasis. Meanwhile，more and more findings illustrate a direct link between the EMT and the gain of stem-cell properties. The purpose of this review is to discuss the relationship between OCSCs and EMT，and to explore the potential therapeutic strategies of ovarian cancer. Related Articles | Metrics

Select

Progress of MIG6 on Progesterone Activity Modulation and Tumor Suppression XU Wei;SHI Xiu;DAI Hui-hua;WANG Xiu-li 2014, 41 (6):  596-598.  Abstract ( 1183 )   PDF (427KB) ( 4827 )   Mitogen-inducible gene 6（MIG6） is induced by epidermal growth factor receptor（EGFR） feedback inhibition factor, can regulate receptor activation and stability. MIG6 is the target gene of progesterone can adjust the antagonistic effect of progesterone on estradiol. MIG6 also possesses the tumor suppressor function. Down-regulation or loss of MIG6 expression is often associated with progesterone resistance of endometriosis（EMs） and the incidence of endometrial cancer. The aim of this review is to highlight recent findings regarding the mechanism of feedback inhibitor of MIG6 on the EGFR signaling pathways and the relationship between MIG6 and progesterone activity，progesterone resistance of EMs and the incidence of endometrial neoplasms. Related Articles | Metrics

Select

Relationship between Estrogen Receptor and Its Variants with Endometrial Cancer HUA Teng;WU Ting-ting;WANG Hong-bo 2014, 41 (6):  599-602.  Abstract ( 1177 )   PDF (505KB) ( 4837 )   The classical estrogen receptor（ER） and its variants，membrane estrogen receptor（mER） involved in mediating different signaling effects of estrogen. Studies have shown estrogen is closely related to the occurrence and development process of endometrial cancer，while excessive estrogen stimulation and lack of progesterone antagonist is widely accepted as the main cause of endometrial cancer and the function of estrogen is mediated by estrogen receptor. Learning the possible mechanisms of the occurrence and development of endometrial cancer and finding new ways for its diagnosis and treatment through estrogen receptor also showing great significance. This paper reviews the structure and function of estrogen receptor，and analyses its relationship with endometrial cancer. Related Articles | Metrics

Select

Effect and Mechanism of Metformin in Endometrial Adenocarcinoma Patients with Metabolic Syndrome NI Juan;SHOU Hua-feng;LOU Han-mei 2014, 41 (6):  603-605.  Abstract ( 1237 )   PDF (411KB) ( 4836 )   Metformin as the first-line drugs, play an important role in the treatment of diabetes. Metabolic syndrome（MS） is a pathological condition of abnormal aggregation of multiple metabolite resulting. The long-term adverse outcomes were payed more and more attention by people. The study found, MS is recognized risk factor of endometrial adenocarcinoma, there may be a common pathophysiological basis of insulin resistance. Metformin reduces insulin resistance, regulates the level of cytokines and exerts anti-tumor stem cell function, it is a potent inhibitor of metabolic syndrome and endometrial adenocarcinoma. Related Articles | Metrics

Select

The Role of Neoadjuvant Chemotherapy in the Treatment of Cervical Cancer CHEN Qi;ZHANG Hong 2014, 41 (6):  606-609.  Abstract ( 1273 )   PDF (542KB) ( 4796 )   The concept of neoadjuvant chemotherapy was first proposed in 1983. Currently，although there is argument in the role of neoadjuvant chemotherapy in the treatment of cervical cancer，the potential advantage of neoadjuvant chemotherapy is apparent gradually by the development of the operation skills and methods. It can effectively reduce tumor volume，increase the success rate of operation，and it can also delay the delivery until fetal viability has been reached. Neoadjuvant chemotherapy is one of the new therapeutic method for locally advanced cervical cancer，advanced cervical cancer and cervical cancer patients during pregnancy. In this essay，the concept，argument，mechanism and the role of neoadjuvant chemotherapy in the treatment of cervical cancer were reviewed. Related Articles | Metrics

Select

Progress in Fertility Sparing Surgery in Young Women with Cervical Cancer WANG Yu;BAI Jing;LI Ai-jun 2014, 41 (6):  610-612.  Abstract ( 1135 )   PDF (631KB) ( 4799 )   In recent years, young women patients with cervical cancer（age less than 35 years old） incidence reports showed an upward trend year by year. At present, the tumor treatment principle is to prolong the survival time and improve the quality of life of patients. For young patients with cervical cancer, the quality of life has become one of the most important influence factors for the choice of treatment，especially for the patients with fertility requirement. This makes the cervical cancer fertility sparing operation has become a new hot spot in medical field. In this paper，we review the new advances of the young women with cervical cancer etiology，pathological type，clinical characteristics and their productive preservation operation in recent years，in order to provide the basis to evaluate the prevention, the diagnosis, the treatment and the prognosis of young women with cervical cancer. Related Articles | Metrics

Select

The Relationship between Human Papillomavirus and Cervical Intraepithelial Neoplasia Recurrence WANG Ya-hui;TONG Jin-yi 2014, 41 (6):  613-616.  Abstract ( 1182 )   PDF (545KB) ( 4794 )   Cervical intraepithelial neoplasia （CIN） is a precancerous change，which can be divided into grade one，grade two and grade three. The disease should be taken to surgery when the pathologic change is more serious than CINⅠ. But there is still some postoperative recurrence rate. There are plenty of factors affecting the recurrence rate，including positive margins，positive endocervical curettage，reduced immunity，senium and so on. Until now，the relationship between HPV and the recurrence rate is not clear，but it′s confirmed that the persistent infection with HPV must increase the recurrence rate. Furthermore，the load of HPV and subtypes of HPV have played an important role in increasing the rate of CIN recurrence. This paper described the influence of HPV on CIN recurrence. Related Articles | Metrics

Select

The Current Status of Nanomaterials in Female Pelvic Floor Reconstruction HOU Min;WU Qing-kai 2014, 41 (6):  617-620.  Abstract ( 1093 )   PDF (629KB) ( 4764 )   Pelvic floor dysfunction（PFD） is the inability of the pelvic floor to fulfil its supportive role to the pelvic organs and/or its inability to allow these organs to function normally，including urinary incontinence（UI）， pelvic organ prolapse（POP） and fecal incontinence（FI）. PFD pathogenesis is not clear，the main objective of treatment is to restore the anatomy and function of the pelvic organs，surgery is the most important treatment for PFD. Due to polypropylene mesh erosion and rejection，scholars are still exploring histocompatibility better pelvic floor reconstruction. Nanomaterials can mimic the nanoscale properties of native extracellular matrix and facilitate cell attachment differentiation and proliferation to guide tissue repair and regeneration. In this review， we discuss the classification，properties， nanotechnology， study status， potential applications of nanomaterials on the field of pelvic reconstruction. Related Articles | Metrics

Select

Advances in Molecular Biology Research on Endometrial Polyps HU Xiao-mei;GUAN Zheng 2014, 41 (6):  621-623.  Abstract ( 1155 )   PDF (529KB) ( 4730 )   Endometrial polyps（EP） is one of the most common endometrial diseases. Patients having it may be totally asymptomatic. While some combined abnormal uterine bleeding, infertility，and even malignant conditions，severely reducing the quality of women's life. Therefore, pinpointing the etiology and pathogenesis is the basis for prevention and treatment of diease. At present most scholars believe that the possible causes include abnormal distribution of estrogen receptor and progesterone receptor，partly endometrial proliferation and apoptosis disequilibrium，but the findings are not the same. In addition，abnormal expression of enzyme，cytokine and genetic factors，and so on, may also be involved in the development of its occurrence. All related factors need to be explored seriously under strict quality management and data control. This article reviews the advances in molecular biology research on endometrial polyps. Related Articles | Metrics

Select

Adenomyosis and Infertility Related Factors GAO Yan;SONG Jing-hui 2014, 41 (6):  624-627.  Abstract ( 1045 )   PDF (577KB) ( 4740 )   Adenomyosis(AM) is a common gynecological disease, in recent years, the incidence is rising. Studies on adenomyosis and infertility-related mechanisms has become a hot topic. Although there is no sufficient epidemiological data to prove that there is a genuine relationship between AM and infertility. However, with improved image and therapeutic levels in AM , indirect evidence shows AM with low fertility. There is no specific mechanism for the interpretation of adenomyosis cause of infertility, this article will combine basic pathological anatomy pathophysiology, and endometrial receptivity abnormalities, endocrine abnormalities and other aspects related to adenomyosis′s research progress, to discuss the relationship between AM and infertility factors. Related Articles | Metrics

Select

Application and Research Progress of Conservative Surgery for Adenomyosis MA Rong-li;GUAN Zheng 2014, 41 (6):  628-631.  Abstract ( 1165 )   PDF (660KB) ( 4776 )   The main symptoms of adenomyosis are progressively secondary dysmenorrhoea and may be associated with hypermenorrhoea or menostaxis，affecting the quality of patients′ normal life seriously. Surgical treatment for adenomyosis is of great importance. As more and more young patients desire to preserve their fertility，therefore，the conservative surgery emerged. At present，conservative surgical managements are composed of the triple-flap method，asymmetric longitudinal dissection of the uterus，excisional surgery，excisional surgery united with uterine artery ligated or uterine nerve ablation，hysteroscopic ablation and excision，wedge-shaped resection and so on. Choice of surgical procedure should be based on patients′ age，symptoms，with or without fertility requirements，and the scope of the lesion decision. Related Articles | Metrics

Select

Treatment of Intrauterine Adhesions with Stem Cells CHEN Fang;SUI Long 2014, 41 (6):  632-635.  Abstract ( 1207 )   PDF (540KB) ( 4740 )   Intrauterine adhesion （IUA） is a common gynecological disease which is associated with endometrial lesion. In recent years,with the popularity of uterine cavity operation and hysteroscopy surgery，the incidence of IUA，as well as the detection rate，is increasing year by year，which has become the second largest cause of secondary infertility in women. For the treatment of IUA，mild-to-moderate of it can obtain certain therapeutic effect with traditional treatment，but for severe or widespread lining damage，the endometrial regeneration and functional recovery have become intractable clinical problems. With extensive research in the field of stem cells in regenerative medicine，domestic and foreign scholars have confirmed the presence of endometrial stem cells，which provides a new way for the treatment of IUA. In this paper，we make a review for the progress of stem cells in treatment of intrauterine adhesion. Related Articles | Metrics

Select

The Role of Estrogen Receptor α in Osteoporosis：An Update YIN Hui-fen;DI Wen 2014, 41 (6):  636-638.  Abstract ( 1085 )   PDF (414KB) ( 4653 )   Reduced bioavailability of estrogen increases osteoporosis and fracture risk in postmenopausal women significantly，but the mechanisms by which estrogen regulates bone mass are incompletely understood. In recent years，mouse models with cell-specific deletion of the estrogen receptor α（ERα） have provided novel insights into the function and signaling of ERα which provide opportunity to develop new drugs for preventing and treating osteoporosis. Illuminating the role of ERα in different bone cells，including chondrocyte，osteoclast，osteoblast progenitor cells，osteoblast and osteocyte to figure out the mechanisms of ERα in the pathogenesis of osteoporosis is of great importance to find new targets for osteoporosis. Related Articles | Metrics

Select

The Relevant Research between Lipid-associated Membrane Proteins of Ureaplasma Urealyticum and Toll-like Receptors WANG Yuan-yuan;ZHANG Hong-wen 2014, 41 (6):  639-642.  Abstract ( 1124 )   PDF (690KB) ( 4749 )   Ureaplasma urealyticum （UU） is the most common pathogenic microorganisms isolated from human urogenital tract, belonging to family mycoplasmataceae molicutes class. It is isolated from the patiens′ urinary tract with non-gonococcal urethritis（NGU） by Shperd in 1954，UU can break down the urea and has its specific biochemical structure and function. Most studies showed that when UU concentration was more than 106 copy/mL in the urogenital tract，sole infection of UU can result in cinical diseases，and they contribute to the genesis and development of many clinical diseases. Recent studies confirmed that UU is associated with human papillomavirus（HPV） and human immunodeficiency virus（HIV） infection，and there is a certain relationship with the spread of HIV. Many researches has always been focused on the pathogenicity of UU，but its pathogenesis has not been elucidated. So some with UU related diseases still lack of effective control. In recent years，the role of toll-like receptors（TLRs） in activating the host innate and adaptive immune response become the hot of research，especially infectious diseases. The lipid-assosiated membrane proteins（LAMPs） of ureaplasma urealyticum can activate intracellular signal transduction pathways by TLRs，induced host cell apoptosis，which may be associated with a variety of diseases. To explore the mechanism and illuminate the process are expected to provide new method for the diagnosis and treatment of clinical related diseases. Related Articles | Metrics

Select

The Relationship between Inflammatory Cytokines and Polycystic Ovary Syndrome ZHANG Lin;LI Xiao-ying 2014, 41 (6):  643-645.  Abstract ( 1190 )   PDF (413KB) ( 4733 )   The pathogenesis of PCOS is very complicated，including the genetic factors，environmental factors，inflammatory factors and so on. In recent years，the relationship between inflammatory factors，insulin resistance，obesity and the hypothalamic-pituitary-gonadal axis and its impact on the pathogenesis of polycystic ovary syndrome is the focus of discussion. Inflammatory cytokines could mediate insulin resistance via adipose tissue. Adipose tissue could secrete multiple cytokines. The abnormal expression of cytokines promotes inflammatory process and participates in the process of insulin resistance in PCOS patients. Besides, inflammatory cytokines may be directly involved in the dysfunction of the hypothalamic-pituitary-gonadal axis and result in the abnormal follicular development, maturation and ovulation process in PCOS patients. In genetic factors, there is a certain correlation between the gene polymorphism of inflammatory cytokines and PCOS. Related Articles | Metrics

Select

The Diagnosis and Treatment of Previous Cesarean Uterine Incision Diverticulum XU Xiao-sheng;LA Duan-duan 2014, 41 (6):  646-649.  Abstract ( 1560 )   PDF (577KB) ( 4713 )   Cesarean incision diverticulum is a rare complication after cesarean section，which could cause irregular uterine bleeding，infertility，chronic pelvic pain，or more likely uterine rupture during pregnancy or childbirth. In recent years，as increase of cesarean section rate，improvement in the clinician′s recognition to this disease，development of imaging and minimally invasive technology，more cases have been confirmed. After introduced the pathogenesis，clinical manifestation and diagnosis of uterine scar defect after cesarean section，the article presented the treatment and latest development of this disease. However，additional and large longitudinal samples and multi-center′s randomized controlled trial are definitely needed. In order to reduce the incidence of uterine scar defect，the cesarean section indication should be strictly controlled. Related Articles | Metrics

Select

The Etiology，Diagnosis and Treatment of Peripartum Cardiomyopathy XU Xin-ran;CUI Hong-yan 2014, 41 (6):  650-654.  Abstract ( 1192 )   PDF (676KB) ( 4644 )   Peripartum cardiomyopathy is defined as an idiopathic cardiomyopathy manifested as heart failure due to left ventricular systolic dysfunction toward the end of pregnancy or in five months after delivery when no other causes of heart failure ars found. Peripartum cardiomyopathy occurs in previously healthy woman，with unknown origin. Although the incidence is low，the mortality rate is high，and the prognosis is different. The patients commonly have dyspnea，cough，neck vein distension and edema，some patients even occurred arrhythmia and myocardial infarction. Diagnosis relies mainly on echocardiography and magnetic resonance images. The disease can be treated by positive myodynamia，diuresis，and anticoagulant drugs. In some patients the clinical symptoms and ultrasonic cardiogram quickly improve and return normal；but in other patients，the clinical symptoms rapidly deteriorating and the drug treatment is ineffective，if persistent left ventricular dysfunction develop to chronic heart failure，ventricular assist devices and cardiac transplantation maybe required. Summarize the etiology，diagnosis and treatment management of the disease. Related Articles | Metrics

论著

Select

The Comparative Study of the Effect of Intraperitoneal Perfusion and Intravenous Chemotherapy in Advanced Ovarian Cancer YAN Yu-lan;ZHANG Dong-mei;WANG Ying;JIANG Ming-zhe 2014, 41 (6):  655-657.  Abstract ( 1403 )   PDF (1765KB) ( 4746 )   Objective：Compare with the efficacy of intravenous chemotherapy and intraperitoneal chemotherapy in two different administration methods of advanced epithelial ovarian cancer. Methods：Select 62 patients who were diagnosed advanced epithelial ovarian cancer in the Tianjin NO.5 Central Hospital, the patients were randomly divided into intravenous chemotherapy and intraperitoneal perfusion group. Intravenous drip group scheme：paclitaxel 135 mg/m2 intravenous infusion in the first day，cisplatin after 24 h 75 mg/m2 intravenously. Intraperitoneal perfusion group scheme：paclitaxel intravenous infusion of 135 mg/m2 in the first day，24 h after cisplatin 100 mg/m2 intraperitoneal，and paclitaxel 60 mg/m2 intraperitoneal perfusion in the eighth day. All patients were followed，compared short-term and long-term efficacy and adverse reactions of the two groups patients respectively. Results：The tumor remission rate in intraperitoneal perfusion group was higher than the intravenous drip group, but the difference wasn′t statistically significant（P＞0.05）；in the compasion of the progression-free survival and 2-year survival rates between the two groups，the intraperitoneal perfusion group was significantly higher than that of intravenous infusion group，difference was statistically significant（P＜0.01）；the adverse reaction of intraperitoneal perfusion group was higher, but only in anemia, liver function damage and the incidence of neurotoxicity which the difference between the two groups was significant (P＜0.05), the other adverse reaction incidence rate between the 2 groups was similar. Conclusions：Intraperitoneal perfusion chemotherapy can effectively improve PFS and 2-year survival rates of patients with advanced epithelial ovarian cancer and also improve patient outcomes. Related Articles | Metrics

Select

The Effect of HPV16 E6 on ER Stress-Autophagic Response in the Cervical Cancer WANG Yu-juan;HUO Jun-ling;LI Jing;WANG Zan-hong 2014, 41 (6):  658-662.  Abstract ( 1103 )   PDF (897KB) ( 4741 )   Objective：To investigate the effects of HPV16 E6 on endoplasmic reticulum（ER） stress-autophagic response in the cervical cancer C33A cells. Methods：The eukaryotic expression vector of HPV16 E6 was constructed and transfected via liｐｏｆｅｃｔａｍｉｎｅ ｉｎｔｏ Ｃ３３Ａ ｃｅｌｌｓ． Ｅｘｐｅｒｉｍｅｎｔａｌ ｃｅｌｌｓ ｗas ｃｌａｓｓｉｆｉｅｄ ｉｎｔｏ ３ ｇｒｏｕｐｓ： ｐｃＤＮＡ３．１（－）－ＨＰＶ１６ Ｅ６ ｇｒｏｕｐ，ｐｃＤＮＡ３．１（－） ｇｒｏｕｐ ａｎｄ Ｃ３３Ａ ｇｒｏｕｐ． Fluorescent quantitation－ｐｌｏｙｍｅｒａｓｅ ｃｈａｉｎ ｒｅａｃｔｉｏｎ ｗａｓ ｕｓｅｄ ｆｏｒ ｄｅｔｅｃｔｉｎｇ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ｍＲＮＡ ｏｆ ＨＰＶ１６ Ｅ６， ａｎｄ Ｗｅｓｔｅｒｎ ｂｌｏｔ ｗas ｅｘｐｌｏｒｅｄ ｔｏ ｍｅａｓｕｒｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ｐｒｏｔｅｉｎ ｏｆ ＨＰＶ１６ Ｅ６，Ｂｅｃｌｉｎ １，ＬＣ３Ⅱ ａｎｄ ＧＲＰ７８ ｉｎ ｔｒａｎｓｆｅｃｔｅｄ ｃｅｌｌｓ． Ｆｌｏｗ ｃｙｔｏｍｅｔｒｙ（ＦＣＭ） ｗａｓ ｅｍｐｌｏｙｅｄ ｔｏ ｏｂｓｅｒｖｅ ｔｈｅ ｅｆｆｅｃｔ ｏｆ ｔｒａｎｓｆｅｃｔｉｏｎ ｏｎ ｔｈｅ ａｕｔｏｐｈａｇｉｃ ｏｆ Ｃ３３Ａ， ａｎｄ ｐｒｏｌｉｆｅｒａｔｉｏｎ ｗａｓ ａｎａｌｙｚｅｄ ｂｙ ＭＴＴ ａｓｓａｙ． Ｒｅｓｕｌｔｓ：Ｅｕｋａｒｙｏｔｉｃ ｅｘｐｒｅｓｓｉｏｎ ｖｅｃｔｏｒ ｐｃＤＮＡ３．１（－）－ＨＰＶ１６ Ｅ６ ｗａｓ ｃｏｎｓｔｒｕｃｔｅｄ ｓｕｃｃｅｓｓｆｕｌｌｙ． Ｔｈｅ ｅｕｋａｒｙｏｔｉｃ ｅｘｐｒｅｓｓｉｏｎ ｖｅｃｔｏｒ ｐｃＤＮＡ３．１（－）－ＨＰＶ１６ Ｅ６ ｓｉｇｎｉｆｉｃａｎｔｌｙ ｉｍｐｒｏｖｅｄ ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ ｍＲＮＡ ａｎｄ ｐｒｏｔｅｉｎ ｏｆ ＨＰＶ１６ Ｅ６ ｉｎ Ｃ３３Ａ ｃｅｌｌｓ． Ｔｈｅ ｃｅｌｌ ｐｒｏｌｉｆｅｒａｔｉｏｎｓ ｏｆ ＨｅＬａ ｃｅｌｌｓ ｉｎｃｒｅａｓｅｄ， ａｎｄ ＦＣＭ ｉｎｖｅｓｔｉｇａｔｉｏｎ ｓｈｏｗｅｄ ｔｈｅ ａｕｔｏｐｈａｇｉｃ ｒａｔｅ ｏｆ ｐｃＤＮＡ３．１（－）－ＨＰＶ１６ Ｅ６ ｇｒｏｕｐ ｗａｓ ｈｉｇｈｅｒ ｔｈａｎ ｔｈａｔ ｉｎ ｐｃＤＮＡ３．１（－） ｇｒｏｕｐ ａｎｄ Ｃ３３Ａ ｇｒｏｕｐ （Ｐ＜０．０５）． Ｔｈｅ ｅｘｐｒｅｓｓｉｏｎ ｏｆ Ｂｅｃｌｉｎ １， ＬＣ３Ⅱ ａｎｄ ＧＲＰ７８ ｗｅｒｅ ｓｉｇｎｉｆｉｃａｎｔｌｙ ｉｍｐｒｏｖｅｄ ａｆｔｅｒ ｔｒａｎｓｆｅｃｔｉｏｎ ｗｉｔｈ ｖｅｃｔｏｒ ｐｃＤＮＡ３．１（＋）－ＨＰＶ１６ Ｅ６（Ｐ＜０．０５）． Ｆｕｒｔｈｅｒｍｏｒｅ， ＧＲＰ７８ ｐｒｏｔｅｉｎ ｌｅｖｅｌ ｗａｓ ｒｅｄｕｃｅｄ ａｎｄ ｔｈｅ ａｕｔｏｐｈaｇｉｃ ｒａｔｅ ｗａｓ ｄｅｃｒｅａｓｅｄ ｂｙ ｔｒｅａｔｍｅｎｔ ｗｉｔｈ ＥＲ ｓｔｒｅｓｓ ｉｎｈｉｂｉｔｏｒ． Ｃｏｎｃｌｕｓｉｏｎs：ＥＲ ｓｔｒｅｓｓ－ａｕｔｏｐｈａｇｉｃ ｒｅｓｐｏｎｓｅ ｐｌａｙｓ ａｎ ｉｍｐｏｒｔａｎｔ ｒｏｌｅ ｉｎ ｔｈｅ ｐｒｏｃｅｓｓ ｏｆ ＨＰＶ１６ Ｅ６ ｉｎｄｕｃｉｎｇ ｃｅｒｖｉｃａｌ ｃａｎｃｅｒ． Ｓｏ ｉｔ ｍｉｇｈｔ ｂｅ ｏｎｅ ｏｆ ｔｈｅ ｎｅｗ ｓｔｒａｔｅｇｉｅｓ ｆｏｒ ｇｅｎｅ ｔｈｅｒａｐｙ ｏｆ ｃｅｒｖｉｃａｌ ｃａｒｃｉｎｏｍａ． Related Articles | Metrics

Select

Correlation of DNA Methyctransferase 1 Gene Polymorphisms and Passive Smoking with Cervical Cancer WANG Ling;JIN Ping;SONG Yi;SUN Yuan-peng 2014, 41 (6):  663-666.  Abstract ( 1108 )   PDF (2445KB) ( 4788 )   Objective：To evaluate the possible relationship between DNA methyctransferase 1（DNMT1）gene polymorphisms and passive smoking with cervical cancer in Han population of Gansu province，China． Methods：A case-control study，DNMT1 gene polymorphism were detected by PCR and gene sequencing method in 100 patients of cervical cancer in Gansu province and 100 healthy controls. The relationship between different genotypes，passive smoking conditions and the risk of cervical cancer was analyzed． Results：Passive smoking has increased risk of developing cervical cancer by 2.705 times（P＜0.05）；the frequency of C allele of rs16999593 in cervical cancer patients was significantly higher than that in control group（P＜0.05）．Conclusions：These findings suggest that DNMT 1 gene polymorphism and passive smoking increased risk of cervical cancer. Related Articles | Metrics

Select

Significance of HPV DNA Type Combined with HPV L1 Capsid Protein Detection in the Prognosis of Cervical Lesions YU Ling-ling;HE Lian-zhi 2014, 41 (6):  667-669.  Abstract ( 1188 )   PDF (12402KB) ( 4747 )   Objective：To analyze the relationship between the two methods and pathological results. Methods：Totally 114 patients who had the results of positive high-risk HPV，pathology and thin-prep liquid-based cytology（TCT） test diagnosed of a typical squamous cells of undetermined significance（ASCUS） and above in the First Affiliated Hospital of Wannan Medical College gynecology clinic from January 2011 to July 2012 were analyzed retrospectively. The expression of HPV L1 were detected in cervical lesions. We analyze the relationship between the subtypes of HPV，HPV L1 capsid protein and pathological results. Results：The positive expression rate of HPV L1 was 64.3%（27/42），54.5%（6/11），15.7%（8/51），0（0/10） in cervictis，CINⅠ，CINⅡ/Ⅲ，SCC，HPV L1 capsid protein expression gradually decreased with cervical lesions increased. The most common HPV subtypes among the 114 patients were HPV16（50%），HPV52（14.91%），HPV58（14.04%），HPV18（7.89%），respectively. In the single infection 16 and 18 subtypes of HPV, HPV L1 protein negative rate in L1 protein rate was low expression，but highly expression in 52，58 subtypes. And in cervical tissue infected with HPV52 and HPV58， HPV L1 protein positive expression group finally confirmed by pathology of inflammation was significantly higher than that of negative group（P＜0.05）. Conclusions：The HPV L1 testing is useful in evaluation of cervical lesions and progression risk of cervical dysplasia，the combination of both HPV L1 and HPV DNA detection can be helpful in management and prognostic prediction. Related Articles | Metrics

Select

The Changes of Cardiac Structure and Myocardial Cell in Ovariectomized Rats YANG Mu-kun;CUI Guang-xia;CHEN Xing;XI Si-si;ZHANG Jing;WANG Ke;QIN Li-hua;BAI Wen-pei 2014, 41 (6):  670-673.  Abstract ( 1111 )   PDF (9198KB) ( 4715 )   Objective：To investigate cardiac structure and function，myocardial cell morphology，and myocardial fibrosis in ovariectomized rats. Methods：Female Sprague-Dawley rats were divided into 2 groups，sham-operated group（Sham group） and ovariectomized group（OVX group），each group has 10 rats. The OVX group underwent bilateral oophorectomy. The 2 groups have undergone UCG to evaluate cardiac structure and function. The heart tissue of each rats were taken HE and Masson staining，to evaluate myocardial morphological changes. Results：Compared with the Sham group， left ventricular septal of OVX group was significantly thicker （P＜0.05）；OVX rats′ myocardial cells area increased significantly（P＜0.01）；ovariectomized rats′ cardiac collagen volume fraction and cardiac perivascular collagen area increased significantly （P＜0.05）. Conclusions：In ovariectomized rats，left ventricular septal and posterior wall have been significant thickening，reconstruction change appeared both in myocardial cells and interstitial collagen. Related Articles | Metrics

Select

Effect of Kaempferol on the BaP Induced Apoptosis in Human Chorionic Trophoblast Cells HTR8/SV neo LIAN Li-fen;CHEN Ya-qiong;HOU Hai-yan;CAO Bo;WU Nian 2014, 41 (6):  674-676.  Abstract ( 1080 )   PDF (1540KB) ( 4688 )   Objective：To study the effect of kaempferol on the BaP induced apoptosis in human chorionic trophoblast cells HTR8/SV neo. Methods：10 μmol/L BaP were added 0.01，0.1，1 μmol/L kaempferol jointly deal with human HTR8/SV neo cells 48 h，observed cells growth under the inverted microscope，Gimesa staining and Hochest33342 fluorescent staining observed morphologic changes，determination of apoptosis rate changes by flow cytometry. Results：① Flow cytometry results showed that BaP alone cell viability was （72.58±0.29）%, with 0.01,0.1,1 μmol/L kaempferol the role of cell viability was （84.96±1.34）%,（89.54±1.64）% and （91.01±1.26）%, respectively, compared with BaP alone group, the differences were statistically significant（P＜0.01）. ②BaP alone can cause cell chromatin condensation，apoptotic bodies and other typical apoptotic morphology，dense hyperchromatic nuclei，fragmentation shape. Conclusions：Kaempferol showed a protective effect on BaP induced apoptosis in HTR8/SV neo cells. Related Articles | Metrics

Select

Expression and Clinical Significance of Bcl-2，BAG-1，Ki-67，ER and PR on Premenopausal and Postmenopausal Endometrial Polyps GUO Hui-mei;GAO Xi-huan;LU Ying-yi;FENG Wen-dong;WANG Yan-yan 2014, 41 (6):  677-679.  Abstract ( 1116 )   PDF (423KB) ( 4706 )   Objective：To stduy the expression and clinical significance of Bcl-2，BAG-1，Ki-67，ER and PR on premenopausal and postmenopausal endometrial polyps. Methods：The expression of Bcl-2，BAG-1，Ki-67，ER and PR in 30 cases premenopausal endometrial polyps and 30 cases postmenopausal endometrial polyps tissues were detected by immunohistochemistry. Results：Bcl-2，BAG-1 and Ki-67 were higher expression in endometrial polyps than control group（P＜0.05），but no signification difference among ER and PR expression（P＞0.05）. Ki-67 expression in postmenopausal endometrial polyps was significantly lower than premenopausal endometrial polyps tissues（P＜0.05）. There were no signification difference among Bcl-2，BAG-1，ER and PR expression between premenopausal and postmenopausal endometrial polyps（P＞0.05）. Correlation analysis showed that Ki-67 was positively correlated with PR on postmenopausal endometrial polyps（P＜0.05），and there was no correlation among other groups（P＞0.05）. Conclusions：Bcl-2，BAG-1，Ki-67，ER and PR may play an important role at different period of endometrial polyps pathological changes. Related Articles | Metrics

Select

Study of Levonorgestrel Intrauterine System in the Treatment of Simple Endometrial Hyperplasia XI Teng-teng;CUI Xiu-juan;SUN Yan-na 2014, 41 (6):  680-682.  Abstract ( 1190 )   PDF (1890KB) ( 4744 )   Objective：To study the efficancy and long possible mechanism of Mirena（levonorgestrel intrauterine system）in the treatment of simple endometrial hyperplasia. Methods：Select sixty cases of simple endometrial hyperplasia in outpatient between 2011.11 and 2012.2，we observed the menstrual patterns，the endometrial thickness and uterine arterial blood flow [resistance index（RI），pulsatility index（PI）] by transvaginal ultrasound，pathologic changes of endometrial by endometrial biopsy in 6 months after insertion of mirena. Results：2 patients were removed Mirena due to colporrhagia. Pathological examinations of 58 patients performed 6 months after the insertion showed that endometrial hyperplasia had reversed. The endometrial thickness，RI of uterine artery in 6 months after the insertion were statistically significant，respectively（1.52±0.33） cm，（0.62±0.14） cm（P＜0.05） and 0.64±0.07，0.79±0.08（P＜0.01）；PI was 1.98±0.51，1.96±0.04，the difference was not statistically significant（P＞0.05）. After placing Mirena significantly reduced by the amount，24 cases showed oligomenorrhea，32 cases showed amenorrhea. Conclusions：Mirena is an efficient and simple treatment of simple endometrial hyperplasia. The influence of uterine arterie blood flow is its possible mechanism. Related Articles | Metrics

Select

Karyotype Analysis of 576 Cases of Congenital Malformations HUANG Hong-qian;FEI Dong-mei;OUYANG Lu-ping;LIU Tian-sheng;SUN Wei-jia;ZHENG Chen-guang 2014, 41 (6):  683-685.  Abstract ( 1155 )   PDF (437KB) ( 4754 )   Objective：By means of ultrasound diagnosis of congenital oaf fetal karyotype analysis，we summarizes the relationship between the chromosomal abnormalities and deformed parts and number of deformity， providing the basis for prenatal diagnosis. Methods：From January 2009 to December 2013 in our hospital an amniocentest or cord blood puncture were performed byindications for prenatal diagnosis of congenital malformations，576 cases of chromosome karyotype analysis of fetus,were investigate the relationship between the congenital oaf and chromosomal abnormalities. Results：The success rate of cell culture successfully are 551 cases（95.66%）；chromosomal abnormalities in 90 cases（16.33%），with 63 cases often dyed anomaly of chromosome abnormality （70.00%）；sex chromosome abnormality 25 cases（27.78% of chromosomal abnormality）；triploid in 1 case；marker chromosomes in 1 case. Freak of chromosome abnormalities, the mainly types are cardiac malformation 31 cases（34.44%），skin edema 30 cases（33.33%），abnormal face and neck 21 cases（23.33%），abnormal brain structure 20 cases（22.22%），and the digestive system malformation 11 cases（12.22%）. Conclusions：Congenital oaf has high incidence of chromosomal abnormalities. For ultrasound tips for congenital oaf , prenatal diagnosis ivtervention should be made to avoid the chromosome abnormalities in children with birth. Related Articles | Metrics

Select

Oxytocin Receptor Inhibitors and β-receptor Agonist in the Treatment of Premature Delivery：a Meta-Analysis YANG Guang-qiong;LONG Shu-yu;YANG Pei;YANG Xing-liang;LI Hua-feng 2014, 41 (6):  686-691.  Abstract ( 1122 )   PDF (1275KB) ( 4708 )   Objective：To evaluate and compare the effects and safety of the oxytocin receptor inhibitors and β-receptor agonist in the treatment of premature delivery. Methods：Based on searching the related literatures from the database by computer，we do a comprehensive collection of randomized trial comparing treatments on the oxytocin receptor inhibitor Atosiban and β receptor agonists including Ritodrine，Salbutamol，Terbutaline. Besides，we do Meta-analysis to the clinical research meeting the inclusive criteria，then we use Cochrane Collaboration RevMan 5.0 software for data processing. Results：A total of six literatures has been identified，Atosiban and β-receptor agonist in the extended gestation of 48 h（RR=1.00，95%CI：0.97-1.04，P=0.90），7 d（RR=1.04，95%CI：0.99-1.09，P=0.14），the difference of the above two groups was not statistically significant; There was no significant difference between the Atosiban group and β-receptor agonist group in gestational age at birth（WMD=0.18，95%CI：-0.19-0.55，P=0.34） and the average weight at birth（WMD=-33.89，95%CI：-108.57-40.79，P=0.37）. However，as to tolerability and efficacy，un-delivery within the 48h and requiring no other tocolytic agent（RR=1.07，95%CI：1.01-1.14，P=0.03） and un-delivery within 7 d and needing no other miscarriage drugs（RR=1.25，95%CI：1.14-1.37，P＜0.05） difference of the Atosiban group and β-receptor agonist group was statistically significant; and incidence of side effects in the mother，especially tachycardia has significant difference（RR=0.09，95%CI：0.05-0.17，P＜0.000 01）；differences in the number of discontinuation people due to side effects of maternal between the Atosiban group and β-agonist group was significant（RR=0.08，95%CI：0.05-0.14，P＜0.000 01）. Conclusions：Compared with β-agonist，Atosiban can achieve the similar therapeutic effect in the treatment of premature delivery，however，its tolerance is much stronger than the β-agonist and incidence of side effects in maternal is much less. Related Articles | Metrics

Select

Expression and Significance of Organic Anion Transporting Polypeptides 4A1（OATP4A1），OATP2B1 in Placenta of Intrahepatic Cholestasis of Pregnancy WANG Hui-min 2014, 41 (6):  692-694.  Abstract ( 1176 )   PDF (467KB) ( 4686 )   Objective：To investigate the expression and significance of organic anion transporting polypeptides 4A1（OATP4A1），OATP2B1 in placenta of intrahepatic cholestasis of pregnancy（ICP）. Methods：Twenty pregnant women with ICP were recruited and twenty normal cesarean section patients as control groups. The expression of mRNA was analyzed by real-time PCR. Data were analyzed by the comparative threshold cycle（CT） method and the 2-△CT method was used（△CT=CT gene of interest -CT internal control）. Results：The expression of OATP4A1 mRNA was higher（P＜0.05） and OATP2B1 was significantly lower（P＜0.01） in ICP placenta than in normal placenta. Significantly elevated cholylglycine（CG）′s level of fetal and maternal serum was associated with ICP. Conclusions：The data presented here suggests that up-regulation of transporter OATP4A1 may involve in the pathophysiology of ICP，which cause the disorder of bile acids. The expression of OATP2B1 in placenta was down-regulation in ICP. It may be involved in the disorder of estrogen metabolism in ICP，causing the mother continued high levels of estrogen stimulation and changed sensitivity. Related Articles | Metrics